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. 2022 Aug 21;298:115661. doi: 10.1016/j.jep.2022.115661

Fig. 2.

Fig. 2

SFJDC can excite A2AAR. (A) RAW264.7 cells using CCK-8. (B) SFJDC stimulated TNF-α, IL-1β, and IL-6 mRNA levels in the supernatant of RAW264.7 cells at 250 μg/mL, 500 μg/mL and 1000 μg/mL (C) and (D) RAW264.7 cells using CCK-8. (E), (F) and (G) A2AAR mRNA expression in RAW264.7 cells. (H) Representative A2AAR immunofluorescence image (scale = 50 μm) and A2AAR fluorescence intensity calculation. Compared to the control group, *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001. Compared to the LPS group, #P ≤ 0.05, ##P ≤ 0.01, ###P ≤ 0.001. Each set of data was based on three replicates.