FIGURE 3.

miR-212-3p overexpression inhibited NLRP3/Caspase-1-activated neuronal pyroptosis in vitro post-Aβ1-42 treatment. (A) miR-212-3p expression was detected through RT-qPCR after the AD cell model was established using Aβ1-42 treatment with H19-7 cells; (B) miR-212-3p expression was detected through RT-qPCR after transfection of mimic NC and miR-212-3p mimic; (C) cell viability was assessed through the MTT method; (D) NLRP3, cleaved Caspase-1, and GSDMD-N expressions were determined through Western blot analysis; (E and F) IL-1β and IL-18 levels were measured through Western blot analysis (E) and ELISA (F); cell experiments were conducted 3 times. Data were formalized as mean ± SD. Data in Figures A and B were analyzed using T test, data in Figure C were analyzed using one-way ANOVA, and data in Figures D-F were analyzed using two-way ANOVA, followed by Tukey’s multiple comparison test.