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. 2022 Feb 12;22(4):540–552. doi: 10.17305/bjbms.2021.6723

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Copyright: © The Author(s) (2022)

This work is licensed under a Creative Commons Attribution 4.0 International License

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miR-212-3p negatively regulated SP1 expression. (A) The binding of miR-212-3p and SP1 was predicted by the Jefferson website (https://cm.jefferson.edu/rna22/Interactive/RNA22Controller); (B) the binding relationship of miR-212-3p and SP1 was testified dual-luciferase reporter assay; (C and D) SP1 expression in the hippocampus was detected through RT-qPCR (C) and Western blot analysis (D); (E and F) SP1 expression in H19-7 cells was detected through RT-qPCR (E) and Western blot analysis (F). N=6. Cell experiments were repeated 3 times. Data were formalized as mean ± SD. Data in Figure B were analyzed using two-way ANOVA and data in Figures C-F were analyzed using one-way ANOVA, followed by Tukey’s multiple comparison test.