FIGURE 5.

SP1 promoted BACE1 transcription. (A) The binding of SP1 and BACE1 promoter region was predicted through the JASPAR website (http://jaspar.genereg.net/analysis); (B-C) the binding of SP1 and BACE1 promoter region was testified through ChIP (B) and dual-luciferase reporter assay (C); (D) BACE1 expression in the hippocampus was detected through RT-qPCR; (E and F) SP1 expression in cells was determined through RT-qPCR (E) and Western blot analysis (F) on H19-7 cells which were transfected oe-NC and oe-SP1; (G) BACE1 expression in H19-7 cells was determined through RT-qPCR on Aβ1-42-treated H19-7 cells which were transfected with miR-212-3p mimic and oe-SP1. N=6. Cell experiments were conducted 3 times. Data were formalized as mean ± SD. Data in Figures B and D-F were analyzed using T test and data in Figures C and G were analyzed using two-way ANOVA, followed by Tukey’s multiple comparison test.