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Inhibitory effect on apoptosis and autophagy of ISL-M in MCAO mice model was proved by Nissl staining (A) and immunofluorescence staining of LC3 (B) and TUNEL (C). Record the number of cells in the same fields of view stained with LC3 (D) and TUNEL (E) by image J.
Notes: Data was presented as mean ± SD, n = 3; vs MCAO: **P < 0.01, ***P < 0.001; vs MCAO + 3 mg/kg ISL: ## P < 0.01, ### P < 0.001.
