Figure 1. CD24 deficiency aggravates diet-induced metabolic disorder in mice.

(A-J) Cd24^−/− mice and WT littermates were fed a HFD for 12 weeks.

(A) Body weight of mice in the indicated groups. n = 8 per group.

(B) Representative photographs, inguinal and epididymal fat pads of mice in the indicated groups.

(C) Body composition of mice was detected by DEXA. n = 8 per group.

(D) TC, TG, FFA, LDL-C and HDL-C levels of mice in the indicated groups. n = 8 per group.

(E) Fasting blood glucose levels of mice in the indicated groups. n = 8 per group.

(F) GTT and ITT results of mice in the indicated groups. n = 8 per group.

(G) Representative photographs of livers from mice in the indicated groups.

(H) Liver weight and liver/body weight ratio of mice in the indicated groups. n = 8 per group.

(I) Representative images of H&E staining of liver sections. Scale bar, 100 μm. Graph shows the quantitation of lipid droplet area. n = 8 per group.

(J) Relative mRNA levels of key metabolic genes in the livers of mice in the indicated groups. n = 6 per group.

(K-N) Cd24^−/− mice and littermate controls were fed a CD-HFD for 12 weeks.

(K) Body weight, liver weight and liver/body weight ratio of mice in the indicated groups. n = 9 per group.

(L) Serum levels of ALT and AST of mice in the indicated groups. n = 9 per group.

(M) H&E and Masson’s trichrome staining of liver sections. Scale bar, 100 μm. Graph shows the quantitation of liver fibrosis area. n = 9 per group.

(N) Relative mRNA levels of profibrotic genes in the livers of mice in the indicated groups. n = 6 per group.

Data are mean ± SEM and representative of two or three independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001, unpaired Student’s t-test (C-E, H-N), two-way analysis of variance (ANOVA) (A, F). See also Figure S1.