Figure 2. CD24Fc alleviates obesity-related metabolic dysfunction in mice.

(A-I) WT mice were fed a HFD and concurrently treated with CD24Fc or IgGFc control twice a week for 8 weeks.

(A) Body weight of mice in the indicated groups. n = 8 per group.

(B) Body composition of mice was detected by DEXA. n = 8 per group.

(C) TC, TG, FFA, LDL-C and HDL-C levels of mice in the indicated groups. n = 6 per group.

(D) Fasting blood glucose levels of mice in the indicated groups. n = 6 per group. (E) GTT and ITT results of mice in the indicated groups. n = 8 per group.

(F) Insulin-stimulated phosphorylation of Akt in the livers of mice. Graph shows the quantitation of p-Akt relative to total Akt. n = 3 per group.

(G) Liver weight and liver/body weight ratio of mice in the indicated groups. n = 6 per group.

(H) Representative images of H&E staining of liver sections. Scale bar, 100 μm. Graph shows the quantitation of lipid droplet area. n = 6 per group.

(I) Relative mRNA levels of key metabolic genes in the livers of mice in the indicated groups. n = 6 per group.

(J-M) WT mice were fed a CD-HFD and simultaneously treated with CD24Fc or IgGFc control twice a week for 8 weeks. </p/>(J) Body weight, liver weight and liver/body weight ratio of mice in the indicated groups. n = 8 per group.

(K) Serum levels of ALT and AST of mice in the indicated groups. n = 8 per group.

(L) H&E and Masson’s trichrome staining of liver sections. Scale bar, 100 μm. Graph shows the quantitation of liver fibrosis area. n = 8 per group.

(M) Relative mRNA levels of profibrotic genes in the livers from mice in the indicated groups. n = 6 per group.

Data are mean ± SEM and representative of two or three independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001, unpaired Student’s t-test (B-D, G-M), two-way analysis of variance (ANOVA) (A, E, F). See also Figure S2.