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. 2022 Aug 8;16:862918. doi: 10.3389/fncel.2022.862918

FIGURE 1.

FIGURE 1

Intrahippocampal injections of amyloid-beta peptide (Aβ)-containing brain homogenate induces Aβ seeding in the corpus callosum (CC). (A) Timeline for the Aβ seeding experiments. (B) Representative immunoblot from whole brain extracts of aged 5xFAD and WT mice compared to synthetic (synth.) Aβ (mixture of synthetic Aβ1-40 and Aβ1-42). Immunoblot was probed with the 6E10 antibody. (C) Illustration of the region of interest (CC; indicated in red) used for subsequent image analysis. Abbreviations: Ctx, cortex; CA1, hippocampal CA1 region; CC, corpus callosum. (D) Fluorescence microscopy visualizing Aβ-material (6E10; red) of 4-month-old WT and 5xFAD mice, which were either uninjected (uninj.) or injected with Aβ-containing brain homogenate of an aged 5xFAD mouse (+5xFAD hom.). Dashed line indicates the Aβ seeding area. (E) Quantification of the Aβ load relative to the CC. n = 6–7 mice with n = 5 sections analyzed per animal. Each symbol represents data from one animal. Mean ± SEM, Kruskal–Wallis test followed by Dunn’s multiple comparison test, **p < 0.01. Scale bar 100 μm.