FIG. 3.

Identification of glyceryl-cysteine sulfone in total acid hydrolysates of performic acid-oxidized NlpI. [2-³H]glycerol-labeled NlpI was purified by preparative SDS-PAGE and treated with performic acid at 4°C overnight. Total acid hydrolysates of performic acid-oxidized lipoprotein were then analyzed by high-voltage paper electrophoresis at pH 2.3 to 2.4 along with the indicated standards. The distance of electrophoretic movement (centimeters) from the origin toward the cathode is indicated by the negatively prefixed scale on the abscissa. Additional experimental details are provided in Materials and Methods.