Table 2.

Renal expression of fibrosis pathway genes in transgenic B6-TGFβ and CBAxB6-TGFβ F1 mice at 14 days of age.

Gene symbol	B6 (n=5)	CBAxB6 F1 (n=5)	B6-TGFβ (n=7)	CBAxB6-TGFβ F1 (n=7)	Kruskal-Wallis test
Tgfb1	1.00 ± 0.18	1.05 ± 0.12	0.80 ± 0.17	1.61 ± 0.42abc	p<0.0001
Ctgf	1.00 ± 0.19	0.69 ± 0.13	0.29 ± 0.06	1.88 ± 0.56abc	p<0.0001
Bgn (biglycan)	1.00 ± 0.39	0.37 ± 0.06	1.01 ± 0.23	1.78 ± 0.46abc	p<0.0001
Dcn (decorin)	1.00 ± 0.28	0.43 ± 0.13	1.09 ± 0.27c	1.84 ± 0.43abc	p<0.0001
Mmp2	1.00 ± 0.29	0.40 ± 0.21	0.97 ± 0.19	0.91 ± 0.38	n.s.
Mmp9	1.00 ± 0.33	3.24 ± 0.57a	0.88 ± 0.30c	4.06 ± 1.04ab	p<0.0001
Timp1	1.00 ± 0.53	0.52 ± 0.28	1.39 ± 0.45	108.48 ± 28.57abc	p<0.0001
Timp2	1.00 ± 0.20	0.61 ± 0.08	0.98 ± 0.09	1.53± 0.11c	p<0.05
Timp3	1.00 ± 0.21	1.31 ± 0.24	1.16 ± 0.07	1.35 ± 0.39	n.s.
Tgbr2	1.00 ± 0.13	0.91 ± 0.15	1.03 ± 0.34	1.15 ± 0.16	n.s.
Smad2	1.00 ± 0.34	0.72 ± 0.18	0.86 ± 0.14	0.95 ± 0.39	n.s.
Smad3	1.00 ± 0.08	0.81 ± 0.19	0.89 ± 0.12	1.03 ± 0.23	n.s.
Smad4	1.00 ± 0.28	0.85 ± 0.22	0.97 ± 0.12	0.91 ± 0.24	n.s.
Smad6	1.00 ± 0.47	0.95 ± 0.39	1.12 ± 0.19	1.10 ± 0.60	n.s.
Smad7	1.00 ± 0.10	0.70 ± 0.17	0.86 ± 0.11	1.07 ± 0.24	n.s.

Legend. Renal mRNA expression values of male TGF-β1 transgenic mice and male wild type controls at the age of 14 days. Expression of each gene was normalized to 18S rRNA (Rn18s) using the 2^-ΔΔCt formula. Data are presented as fold expression to a pooled control sample (mean ± SD).

^ap<0.05 vs B6;

^bp<0.05 vs B6-TGFβ;

^cp<0.05 vs CBAxB6 F1.