Figure 1.
Microbial diversity of apple, intercropping plants, and sandy waste soil (SWS). (A) Microbial diversity of different groups according to the ACE index. The lines inside the boxes represent the median values (95% confidence). The significant differences in the alpha indices between groups are indicated by different lowercase letters (p < 0.05). (B) Manhattan distance analysis of bacterial communities from different samples (principal coordinates PCo1 and PCo2). The microbiomes of apple, intercropping plants and SWS were significantly separated from each other along the two axes (P < 0.001, permutational multivariate analysis of variance, PERMANOVA). The ellipses cover 90% of each group. (C,D) Manhattan plots present significantly enriched bacterial operational taxonomic units (OTUs) in the apple vs. licorice (C) and apple vs. SWS (D) comparisons. (E) Relative abundance of the predominant bacteria (at the phylum level) in different samples. APR, root soil of apple plant; MG, root soil of licorice; NG, root soil of natural grass; MP, root soil of pepper plant; SWS, soil of sandy waste.