Figure 4.

CFA treatment results in DNA hypomethylation and unchanged DNMT3a levels in the amygdala. (a–c): DNA methylation levels in amygdala (AMY, a), ventral hippocampus (VHPC, b) and prefrontal cortex (PFC, c) 10 days following the intraplantar administration of 50 μL CFA or 0.9% sterile saline in the right hind paw. Each sample result is expressed as 5-methyl-2-deoxycidine/total DNA normalized by the 5-methyl-2-deoxycidine/total DNA of the mean of the control group and expressed as percentage. (d–f): Quantification of DNMT3a levels (relative to actin) in the amygdala (d), ventral hippocampus (e) and prefrontal cortex (f) 10 days after CFA or saline administration. Representative bands can be seen on the upper side. N = 3–6/group. Results are expressed as mean ± the mean standard error (SEM) and were analyzed by Student t test. *p < .05. Image illustrated using Biorender© software. CFA, Complete Freund’s adjuvant.