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. 2022 Aug 8;13:936995. doi: 10.3389/fimmu.2022.936995

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Copyright © 2022 Royzman, Andreev, Stich, Peckert-Maier, Wild, Zinser, Mühl-Zürbes, Jones, Adam, Frey, Fuchs, Kunz, Bäuerle, Nagel, Schett, Bozec and Steinkasserer

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Downstream of TLR4, sCD83 acts via the TRIF-mediated signaling cascade in a MyD88-independent manner. (A) The mRNA levels of Oscar, Dc-stamp, and Mmp9 were analyzed via RT-PCR, and Rpl4 was used as a housekeeping gene. Relative expression of sCD83-treated cultures was normalized to the corresponding phosphate-buffered controls (PBS) (with n = 3 each). (B) Representative images (upper side) used for the quantification of large multinucleated TRAP⁺ cells with more than 15 nuclei (lower side) generated from nucleofected cells, where MT-1 and MT-2 were knocked down by CRISPR/Cas9 with n = 3, respectively. Panel (A) was tested for significance using ordinary one-way ANOVA against the control condition (black) and panel (B) by Student’s t-test.