Table 1.

Targeting sequences used for CRISPR/Cas9-mediated gene knockouts as predesigned by IDT’s guide RNA tool (first two columns) along with specific primers which were used in order to determine the knockout efficiency (last three columns, 5′ → 3′).

Gene	Target sequence		Forward	Reverse
CD83	GCTGCAACTCGGGGACATAC	huCD83	ATCCAGCAAGATTCACACACAGC	CGGATTTCCACTTTCAGCCACA
TLR4	TCTGACGAACCTAGTACATG	muTLR4	CCAGCCAGGTTTTGAAGGCAA	GTATCTCTTTTGCCCATAGGTGTGA
TRIF/TICAM1	CAAGCTATGTAACACACCGC	muTRIF/TICAM1	CTCGTCCCATTGACACACCA	AGGGGAGACCTGGAGTTTGT
MyD88	TCCCACGTTAAGCGCGACCA	muMyD88	AACTCCACAGGCGAGCGTA	TGGAAGGGGTCCTCACTTGT
MT-1	TCGTCCAACGACTATAAAGA	muMT-1	CTCTCTGGAGGGAGGGTACC	AGCCTCTACAACTCGGGGAT
MT-2	GCGTGATGGAGAGAAGCACG	muMT-2	TGTGCTGGCCATATCCCTTG	GTCGGAAGCCTCTTTGCAGA