FIG. 10.

Analysis of DNA binding activity of ³⁵S-labeled Orf10 purified from inclusion bodies. The binding reaction was carried out as described in Materials and Methods, and products were analyzed by native PAGE and autoradiography. Lanes: 1, in the absence of DNA; 2, with EcoRI-digested pUC19; 3, with EcoRI-digested M13mp18; 4, with the 246-bp NruI-AvaI fragment within the orf10-orf11 intergenic region (pCNB033 insert); 5, with the 206-bp AhaII fragment within the orf10-orf11 intergenic region (pCNB034A insert); 6 and 7, with the 393-bp PstI-SacII and 227-bp SmaI-HincII fragments containing direct repeat sequences at the 3′ ends of orf10 and orf11, respectively; 8, with the 484-bp EspI-XhoI fragment within the orf10-orf11 intergenic region containing the NdeI-engineered restriction site; 9, with the 455-bp MboII fragment within the actIII-actI intergenic region. Arrows indicate mobilities of the complexes between Orf10 protein and its DNA target.