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Subcloning of the orf10-orf11 DNA region to elucidate the genes required for complementation analysis of actIII mutation. Plasmid construction was as described in Materials and Methods and Table 1. Plasmids pSCNB01 and pSCNB010 contain a TAG stop codon (generated by the cloning procedure) downstream of the 3′ end of the orf10 deletion. Plasmid pIJ2314 contains the actIII gene. Symbols: −, no actinorhodin production; +, actinorhodin production.
