FIG. 7.

SDS-PAGE analysis of orf10 expression in E. coli and at various stages of its purification. Lanes: 1 and 2, whole-cell extracts from cultures of E. coli K12ΔH1Δtrp carrying the vector plasmid pAZe3ss, grown at 30 and 42°C, respectively; 3 and 4, whole-cell extracts from cultures of the same strain harboring plasmid pCNB019, grown at 30 and 42°C, respectively; 5, supernatant of 25,000 × g centrifugation; 6, pellet of this centrifugation (inclusion bodies) after the refolding step; 7, heparin-agarose chromatography (1 μg of protein). The recombinant purified Orf10 protein is indicated by arrowheads. The positions and molecular masses of marker proteins are shown on the left.