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Map of suicide vector pNHG1. The plasmid encompasses 8,810 bp and was constructed by inserting a fragment carrying the tetracycline resistance gene tet from pBBR1MCS-3 into pLO1. Only unique cleavage sites of restriction endonucleases are indicated within the multiple cloning site (MCS). neo, the gene encoding kanamycin resistance; oriT, the origin of transfer replication; sacB, the gene encoding levansucrase.
