TABLE 3.

Activities of mutant P_L promoters determined by P_L::lacZ transcriptional fusions in transconjugants of R. eutropha H16

Transconjugant	Sp act of β-galactosidase (mU/mg of protein)a grown on:
	H2-CO2	Fructose	Pyruvate
pBKb	4	3	3
pBK2241c	930	100	6
pBKM1d	1,590	1,200	90
pBKM2	1,120	560	16
pBKM3	930	360	8
pBKM4	5	5	6
pBKM5	1,770	300	16
pBKM6	1,940	1,440	90
pBKM7	1,580	240	12
pBKM8	15	7	5
pBKM9	2,200	1,610	1,270
pBKM10	1,300	320	24
pBKM11	1,580	120	6
pBKM12	40	9	6
pBKM13	320	70	6
pBKM14	1,140	100	6

^aThe activity values represent mean values obtained from at least three independent determinations. They varied up to ±5 mU/mg in the low-level activity range (3 to 40 mU/mg), up to ±20 mU/mg in the intermediate range (70 to 360 mU/mg), and up to ±100 mU/mg in the high range (930 to 2,200 mU/mg). 

^bTransconjugant H16(pBK) served as a background reference. 

^cpBK2241 contains wild-type P_L fused to lacZ and P_R fused to gusA. 

^dpBKM1 through pBKM14 contain mutant P_L M1 through M14, respectively.