TABLE 4.

Activities of mutant P_L promoters determined by P_L::lacZ transcriptional fusions in transconjugants of R. eutropha

Transconjugant	Sp act of β-galactosidase (mU/mg of protein)a for:
	Fructose + formate				Pyruvate
	H16	HB14	HF210	HB15	H16	HB14	HF210	HB15
pBKb	4	3	3	2	3	3	3	4
pBK2241c	780	6	490	5	6	4	6	2
pBKM1d	1,980	160	1,100	130	90	60	60	40
pBKM6	1,570	170	1,590	70	90	60	40	30
pBKM9	1,800	1,150	1,630	790	1,270	860	730	480

^aThe activity values represent mean values obtained from at least three independent determinations. They varied up to ±5 mU/mg in the low-level activity range (2 to 40 mU/mg), up to ±10 mU/mg in the intermediate range (60 to 170 mU/mg), and up to ±100 mU/mg in the high range (480 to 1,980 mU/mg). 

^bTransconjugant H16(pBK) served as a background reference. 

^cpBK2241 contains wild-type P_L fused to lacZ and P_R fused to gusA. 

^dpBKM1, pBKM6, and pBKM9 contain P_L M1, M6, and M9, respectively.