TABLE 5.

P_R promoter activities associated with the mutant P_L promoter M1, M6, or M9 and determined by P_R::gusA transcriptional fusions in transconjugants of R. eutropha

Transconjugant	Sp act of β-glucuronidase (μU/mg of protein)a for:
	Fructose + formate				Pyruvate
	H16	HB14	HF210	HB15	H16	HB14	HF210	HB15
pBKb	4	4	6	4	6	9	3	9
pBK2241c	480	2,990	40	50	18	240	30	8
pBKM1d	2,750	3,430	7,020	7,170	910	900	990	990
pBKM6	160	290	70	120	6	10	35	12
pBKM9	150	390	80	170	20	10	50	17

^aThe activity values represent mean values obtained from at least three independent determinations. They varied up to ±5 μU/mg in the low-level activity range (3 to 40 μU/mg), up to ±50 μU/mg in the intermediate range (50 to 480 μU/mg), and up to ±300 μU/mg in the high range (900 to 7,170 μU/mg). 

^bTransconjugant H16(pBK) served as a background reference. 

^cpBK2241 contains wild-type P_L fused to lacZ and P_R fused to gusA. 

^dpBKM1, pBKM6, and pBKM9 contain P_L M1, M6, and M9, respectively, fused to lacZ and P_R fused to gusA.