Fig. 1.

Effect of fisetin on phosphorylation of YB-1 and AKT is cell line dependent. The TNBC cells were treated with indicated concentrations of fisetin for 24 h. Thereafter, protein samples were extracted and loaded into a SDS-PAGE. The level of phosphorylation of YB-1 (S102) and AKT (S473) were detected by Western blotting. Blots were stripped and incubated with antibody against YB-1 and AKT1, respectively. Actin was detected from the YB-1 detected membrane without stripping as a loading control. The histograms represent the mean densitometry values ± SD of phospho-YB-1 to actin, YB-1 to actin and phospho-AKT to AKT1 normalized to 0 µM condition from 3 independent experiments. The asterisks indicate significant fisetin mediated changes on YB-1 and AKT phosphorylation (*p < 0.05, **p < 0.01, ***p < 0.001), ****p < 0.0001; students t-test). SD: standard deviation