FIG. 8.

Localization and expression levels of CglB antigen determined by PAGE and Western blot analysis using polyclonal anti-CglB antiserum. (A) Cell extract (lane 2) of DK1622 was separated into cytoplasmic (lane 3) and membrane (lane 4) fractions. Only a single 44-kDa band cross-reacted with the anti-CglB antibody in the total-cell extract and in the membrane fraction. Lanes 2 to 4 contain about 20 μg of protein. Lane 1, size markers. (B) Expression levels of CglB in DK1622 (lane 1), ASX21 (lane 2), DK1622 (lane 3), and ASX31 (lane 4). Each lane contains about 12 μg of cellular protein.