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. 2022 Aug 22;4(3):lqac058. doi: 10.1093/nargab/lqac058

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© The Author(s) 2022. Published by Oxford University Press on behalf of NAR Genomics and Bioinformatics.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 3. — Designed peptides specifically bind to the S protein in sandwich ELISA. (A) Schematic of a sandwich ELISA where the designed peptides are used to both capture (SS2 peptide recognize the S1/S2 region) and detect (R1 peptide recognize the RBD region) the S protein from a mixture with cell lysates. Represented in (B) the sandwich ELISA can detect the presence of the S protein in the cell lysate mixtures down to 100 pg per ml (0.1 ng/ml). Each experiment is repeated at least four times, independently.