Table 2.

Overview of Ampli1‐amplified HBD samples obtained to calibrate the bioinformatic pipeline. Samples are grouped per donor. Checkmarks indicate HBD leukocytes used in the calibration set. VyCAP QC: scores can range from QC‐0 to QC‐7. Ampli1 QC: scores can range from QC‐0 to QC‐4. No, number.

Sample No.	Sample Name	Sample Type	Donor/Source	Gender	Input	VyCAP QC score	Ampli1 QC score	Yield TruSeq (nm)	Used in calibration set
25	HBD1‐1	Leukocyte	HBD1	Female	1 cell	7	2	2.8	✔
26	HBD1‐2	Leukocyte	HBD1	Female	1 cell	6	3	3.8
27	HBD1‐3	Leukocyte	HBD1	Female	1 cell	6	2	2.0
28	HBD1‐4	Leukocyte	HBD1	Female	1 cell	7	4	3.3
29	HBD2‐1	Leukocyte	HBD2	Male	1 cell	7	4	2.9	✔
30	HBD2‐2	Leukocyte	HBD2	Male	1 cell	7	4	3.4
31	HBD2‐3	Leukocyte	HBD2	Male	1 cell	7	4	4.6
32	HBD2‐4	Leukocyte	HBD2	Male	1 cell	7	4	3.4	✔
33	HBD3‐1	Leukocyte	HBD3	Female	1 cell	7	4	3.9	✔
34	HBD3‐2	Leukocyte	HBD3	Female	1 cell	7	4	3.5
35	HBD3‐3	Leukocyte	HBD3	Female	1 cell	5	3	2.1
36	HBD3‐4	Leukocyte	HBD3	Female	1 cell	7	4	2.2	✔
37	HBD4‐1	Leukocyte	HBD4	Male	1 cell	7	1	8.6
38	HBD4‐2	Leukocyte	HBD4	Male	1 cell	7	3	6.4	✔
39	HBD4‐3	Leukocyte	HBD4	Male	1 cell	7	4	2.9	✔
40	HBD4‐4	Leukocyte	HBD4	Male	1 cell	7	4	5.8	✔
41	HBD5‐1	Leukocyte	HBD5	Male	1 cell	7	2	7.5	✔
42	HBD5‐2	Leukocyte	HBD5	Male	1 cell	7	3	10.2	✔
43	HBD5‐3	Leukocyte	HBD5	Male	1 cell	7	4	10.9
44	HBD5‐4	Leukocyte	HBD5	Male	1 cell	7	4	9.9
45	HBD6‐1	Leukocyte	HBD6	Female	1 cell	6	4	6.6	✔
46	HBD6‐2	Leukocyte	HBD6	Female	1 cell	7	3	10.0	✔
47	HBD6‐3	Leukocyte	HBD6	Female	1 cell	7	4	5.8
48	HBD6‐4	Leukocyte	HBD6	Female	1 cell	6	4	10.5	✔