Fig. 5.

Effects of other commensal bacterial strain on specialized proresolving mediator (SPM) biosynthetic machinery and angiogenic potential in colorectal carcinoma (CRC) cells. (A) ALOX5, ALOX15A and ALOX15B protein expression levels (mean fluorescence intensity), assessed by cytofluorimetric analysis, in HCT116 cells treated or not (NT) with Escherichia coli (E. coli), Bifidobacterium bifidum (B. bifidum) and Lactobacillus rhamnosus GG (LGG) supernatant (SN) (1 : 30 titration) for 6 h. Data are represented as mean ± SD of three independent experiments. *P < 0.05 compared with the relative control by Student's t test. (B) RvD1 release from HT29 and HCT116 cells treated or not (NT) for 12 h with E. coli, B. bifidum and LGG SN (1 : 30 titration). Data are represented as mean ± SD of three independent experiments. *P < 0.05 compared with the control by Student's t test. (C) VEGF‐A release from HT29 and HCT116 cells treated or not (NT) for 12 h with E. coli, B. bifidum and LGG SN (1 : 30 titration). Data are represented as mean ± SD of three independent experiments. *P < 0.05 compared with the control by Student's t test. [Colour figure can be viewed at wileyonlinelibrary.com]