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Primer sequences and annealing temperatures used for RT-qPCR.
| Gene | GenBank Accession No. |
Primer Sequence (5′-3′) | Product Size (bp) | Annealing Temperature (°C) |
|---|---|---|---|---|
| Lef1 | XM_042251154.1 | F: ACCATGACAAGGCCAGAGAA | 228 | 60 |
| R: TGATGAGAGGGGTGAGAGGA | ||||
| Dlx3 | XM_004012779.5 | F: AACCGCCGTTCCAAATTCAA | 230 | 60 |
| R: TGTGCATGGTACCAGGAGTT | ||||
| β-actin | NM_001009784 | F: AGCCTTCCTTCCTGGGCATGGA | 113 | 60 |
| R: GGACAGCACCGTGTTGGCGTAGA |
