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. 2022 Jul 26;12(8):691. doi: 10.3390/metabo12080691

Figure 3.

Figure 3

Detection and identification of secondary metabolites by UHPLC–HRMS. (A) TIC (total ion chromatogram) of metabolites in different varieties and stages of coconut flesh obtained from UHPLC–MS/MS analysis. (B) EIC (extracted ion chromatogram) of apigenin 5-O-glucoside and apigenin 7-O-glucoside detected at 4.28 min and 4.35 min in sample. Apigenin 7-O-glucoside detected at 4.35 min in standard. (C) MS/MS spectra at m/z 433.11124, and the metabolite was identified as apigenin 7-O-glucoside. (D) The molecular structure of the apigenin 7-O-glucoside and apigenin 5-O-glucoside and their general fragmentation rules.