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. 2022 Jan 14;12(4):1070–1087. doi: 10.1158/2159-8290.CD-21-0808

Figure 1.

Figure 1. CC supplementation is associated with antitumor activity that is microbiome dependent and circumvents the αPD-1 resistance in mice conferred by FMT from NR patients with NSCLC. A and B, Tumor growth kinetics in SPF C57BL/6 mice after sequential injections of αPD-1 or IsoPD-1 and daily oral gavage with CC or water are depicted for (A) MCA-205 sarcoma (n = 15 mice/group) and (B) E0771 breast cancer (n = 10 mice/group). C, Experimental design of avatar mice experiments. FMT from feces samples from NR and R patients with NSCLC were individually performed in GF C57BL/6 mice or after 3 days of ATB in SPF C57BL/6 mice. Two weeks later, MCA-205 tumors were inoculated, and daily gavage with CC or water was performed in combination with sequential injections of αPD-1 or IsoPD-1. D, Pooled analysis of the mean tumor size ± SEM at sacrifice post-FMT from four NR (NR1–2, 4–5) and four R (R1–4) groups for each CC and water group. Each symbol represents one mouse. E, MCA-205 tumor kinetics in mice reared in GF conditions and receiving daily oral gavage with CC or water (n = 5 mice/group). F, Tumor size at sacrifice of mice bearing MCA-205 treated with αPD-1 or IsoPD-1 in combination with daily FMT with mouse feces previously supplemented with CC. Each circle represents one mouse. Means ± SEM are represented in all experiments. ns, nonsignificant; *, P < 0.05; **, P < 0.01; ***, P < 0.001.

CC supplementation is associated with antitumor activity that is microbiome dependent and circumvents the αPD-1 resistance in mice conferred by FMT from NR patients with NSCLC. A and B, Tumor growth kinetics in SPF C57BL/6 mice after sequential injections of αPD-1 or IsoPD-1 and daily oral gavage with CC or water are depicted for (A) MCA-205 sarcoma (n = 15 mice/group) and (B) E0771 breast cancer (n = 10 mice/group). C, Experimental design of avatar mice experiments. FMT from feces samples from NR and R patients with NSCLC were individually performed in GF C57BL/6 mice or after 3 days of ATB in SPF C57BL/6 mice. Two weeks later, MCA-205 tumors were inoculated, and daily gavage with CC or water was performed in combination with sequential injections of αPD-1 or IsoPD-1. D, Pooled analysis of the mean tumor size ± SEM at sacrifice post-FMT from four NR (NR1–2, 4–5) and four R (R1–4) groups for each CC and water group. Each symbol represents one mouse. E, MCA-205 tumor kinetics in mice reared in GF conditions and receiving daily oral gavage with CC or water (n = 5 mice/group). F, Tumor size at sacrifice of mice bearing MCA-205 treated with αPD-1 or IsoPD-1 in combination with daily FMT with mouse feces previously supplemented with CC. Each circle represents one mouse. Means ± SEM are represented in all experiments. ns, nonsignificant; *, P < 0.05; **, P < 0.01; ***, P < 0.001.