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. 2022 Feb 8;12(5):1233–1247. doi: 10.1158/2159-8290.CD-21-1119

Figure 1.

Figure 1. Zeno inhibits growth and blocks signal transduction in cell lines with NRG1 fusions. A–C, Cells were treated with the indicated concentrations of Zeno for 96 hours and then growth was determined using AlamarBlue viability dye. Values are expressed relative to the vehicle-treated control (100%). Data were analyzed by nonlinear regression to determine IC50 for inhibition of growth (see Supplementary Fig. S2A for IC50 values). Results represent the mean ± SD of three replicate determinations in one experiment. D–F, For Western blot analyses, cells were deprived of serum for 24 hours and then treated with the indicated concentrations of Zeno for 1.5 hours prior to preparation of whole-cell extracts and immunoblotting. Representative immunoblots are shown with GAPDH expression used as a western blotting loading control. At least two independent experiments were conducted.

Zeno inhibits growth and blocks signal transduction in cell lines with NRG1 fusions. A–C, Cells were treated with the indicated concentrations of Zeno for 96 hours, and then growth was determined using AlamarBlue viability dye. Values are expressed relative to the vehicle-treated control (100%). Data were analyzed by nonlinear regression to determine IC50 for inhibition of growth (see Supplementary Fig. S2A for IC50 values). Results represent the mean ± SD of three replicate determinations in one experiment. D–F, For Western blot analyses, cells were deprived of serum for 24 hours and then treated with the indicated concentrations of Zeno for 1.5 hours prior to preparation of whole-cell extracts and immunoblotting. Representative immunoblots are shown, with GAPDH expression used as a Western blotting loading control. At least two independent experiments were conducted.