FIG. 1.
(A) Restriction map of the M. pneumoniae P65 operon (crl) region. Open reading frames encoding P65, HMW2, P41, and P24 (orfp65, hmw2, orfp41, and orfp24, respectively) are indicated. The EcoRI fragment used in Southern blot hybridizations is indicated by the bar above the map, and the arrow denotes the predicted transcriptional initiation site based on primer extension studies (21). (B) The recombinant Tn4001mod transposons engineered to contain the PstI-BglII or BstEII-BglII fragment from the P65 operon in the SmaI site of IS256L. Plasmids containing the various recombinant transposons are indicated on the right, and the arrows above each indicate the orientation of the cloned fragment, relative to those of the Pin and Pout promoters. Bs, BstEII; Bg, BglII; E, EcoRI; P, PstI; S, SmaI; Sc, ScaI.