Figure 1.

Vaccine-induced antibody responses. BALB/cJRj mice were immunized with 2x10⁸ particles Ad-F and 1x10⁹ particles Ad-empty, Ad-IPS-1, or Ad-IL-1β. Antibody levels were characterized in sera 35 days and in BAL samples 42 days post-immunization. (A, B) FACS-based analysis for F-specific antibodies was performed for Ig in sera (A, 1:100 dilution, detection with anti-Ig-FITC) and for IgA in BAL samples (B, 1:20 dilution, detection with anti-IgA-FITC) with HEK 293A cells expressing the F protein. Shown are the MFIs for each sample and the mean of the values for each group. (C) 75% plaque reduction neutralization titers (PRNT75) were analyzed in sera by RSV microneutralization assay (detection limit at a titer of <1:12). Bars represent group means (A, B) or medians (C) overlaid with individual data points. n=10-11 for sera and n=4-5 for BALs. Data were analyzed by one-way ANOVA followed by Tukey´s post test (values in C were log-transformed before analysis). *p<0.05, ** p<0.005, *** p<0.0005, **** p<0.0001.