Figure 2.

Vaccine-induced T cell responses in the lung. BALB/cJRj mice were immunized as described above and lung lymphocytes were analyzed 49 days later. Counts of F_85-93 pentamer⁺ CD8⁺ T cells per lung (A) and counts of specific memory T cell subsets (B) are shown; effector T cells, T_EFF, KLRG1⁺CD127^-; effector memory T cells, T_EM: KLRG1⁺CD127⁺; central memory T cells, T_CM: KLRG1^-CD69^-CD103^-CD127⁺; T_RM: KLRG1^-CD127^+/-CD69⁺CD103⁺; gating scheme in Supplementary Figure 2 . (C, D) Lung lymphocytes were restimulated with MHC-class I and class II peptides derived from the F protein and the functionality of CD8⁺ T cells (C) and CD4⁺ T cells (D) was detected via extracellular staining for CD107a and intracellular cytokine staining (poly; for CD8⁺: CD107a⁺IFNγ⁺IL-2⁺TNFα⁺; for CD4⁺: IFNγ⁺IL-2⁺TNFα⁺; gating strategy in Supplementary Figure 3 ). Bars represent group means overlaid with individual data points. n=4-5. Data were analyzed by one-way ANOVA followed by Tukey´s post Test. *p<0.05, ** p<0.005, *** p<0.0005, **** p<0.0001.