Figure 4.

Humoral responses induced by mucosal vaccination or primary RSV infection. BALB/cJRj mice were infected with 5x10⁵ PFU RSV-A2 or immunized with 2x10⁸ particles Ad-F and 1x10⁹ particles Ad-IL-1β. Antibody levels were characterized in sera 35 days and in BAL samples 42 days post-immunization. (A, B) Virus-specific IgG1 and IgG2a in sera (A, 1:1000 dilution) and Ig and IgA in BAL (B, 1:10 dilution) were detected via virus-coated ELISA. (C) 75% plaque reduction neutralization titers (PRNT75) in sera (detection limit at a titer of <1:12, dotted line) and BAL (detection limit at a titer of <1:2, dotted line) were tested by microneutralization assay. (D, E) FACS-based analysis for F- (D) and G-specific (E) antibodies was performed in sera (1:100 dilution) and BAL samples (1:20 dilution). Bars represent group medians (A-C) or means (D, E) overlaid with individual data points. n=8-12 for sera and n=4-6 for BALs. Data were analyzed by one-way ANOVA followed by Tukey´s post test (values in A-C were log-transformed before analysis). *p<0.05, ** p<0.005, *** p<0.0005, **** p<0.0001. Relative light units per second, RLU/s.