Figure 5.

T cell phenotypes induced by mucosal vaccination and a primary RSV infection. BALB/cJRj mice were immunized or infected as described above and isolated lung lymphocytes were analyzed seven weeks later. Counts of F_85-93 pentamer⁺ (A) and M2_82-90 pentamer⁺ CD8⁺ T cells (C) per lung and of specific memory T cell subsets (B, D) are shown; effector T cells, T_EFF, KLRG1⁺CD127^-; effector memory T cells, T_EM, KLRG1⁺CD127⁺; central memory T cells, T_CM, KLRG1^-CD69^-CD103^-CD127⁺; T_RM, KLRG1^-CD127^+/-CD69⁺CD103⁺; gating scheme in Supplementary Figure 2 . (E) Pentamer⁺ CD8⁺ T cells (M2_82-90-spec. in RSV group, F_85-93-spec. in Ad-F+Ad-IL-1β group) that were not stained by the intravenous staining were further characterized for their expression of CD11a, CD69, and CD103. Bars represent group means overlaid with individual data points. n=4-6. Data were analyzed by one-way ANOVA followed by Tukey´s post test (A-D) or two-tailed Student’s t-test (E). *p<0.05, ** p<0.005, *** p<0.0005, **** p<0.0001.