Figure 6.

Mucosal T cell functionality induced by mucosal vaccination and a primary RSV infection. Lung lymphocytes were restimulated with MHC-class I peptides derived from the F (A) and the M2 protein (B) and the functionality of CD8⁺ T cells was detected via extracellular staining for CD107a and intracellular cytokine staining (poly; CD107a⁺IFNγ⁺IL-2⁺TNFα⁺; gating strategy in Supplementary Figure 3 ). Bars represent group means overlaid with individual data points. n=4-6. Data were analyzed by one-way ANOVA followed by Tukey´s post Test. *p<0.05, ** p<0.005, *** p<0.0005, **** p<0.0001.