Figure 7.
Protection against RSV infection mediated by vaccination and a primary RSV infection. Mice were experimentally infected with 1x106 PFU RSV-A2 48 days after the immunization or primary infection. (A) The initial weight was set as 100% (dotted line) and weight changes were monitored for five days. (B) Viral RNA copies were quantified by qRT-PCR in lung homogenates five days post-infection. The dotted line represents the lower limit of quantification. (C) Tissue damage was assessed by measuring the total concentration of protein in BAL samples via bicinchoninic acid assay. Data represent group means with SEM (A), group medians overlaid with individual data points (B), or group means overlaid with individual data points (C). n=4-6. Data were analyzed by one-way ANOVA followed by Tukey´s post test (values in B were log-transformed before analysis). For (A): # p<0.05 vs. F+IL-1β. For (B, C): *p<0.05, ** p<0.005, *** p<0.0005, **** p<0.0001.