Table 4.
Bacterial 16S PCR results on plasma samples from bacteraemic and no paired BC subjects at various time points relative to positive blood culture. n.d., Not detected; w.b., weak band.
|
Bacterium/ |
>-30h |
−30h<0<30h |
>30 h |
|||
|---|---|---|---|---|---|---|
|
Subject |
Qubit (ng µl−1) |
16S PCR |
Qubit (ng µl−1) |
16S PCR |
Qubit (ng µl−1) |
16S PCR |
|
Sepi/Scap-2 |
1.79 |
n.d. |
0.54 |
n.d. |
||
|
CNS/Efu-3 |
0.06 |
n.d. |
||||
|
Kpn-3# |
4.98 |
n.d. |
||||
|
Entc-6* |
0.27/0.66† |
n.d./n.d.‡ |
||||
|
Psa-12 |
0.16 |
n.d. |
0.25 |
n.d. |
||
|
Spn-13 |
0.5 |
n.d. |
2.28 |
n.d. |
||
|
MRSA/MSSA-16 |
1.16/0.24† |
n.d./n.d.‡ |
||||
|
No-paired BC-17* |
0.08 |
n.d. |
||||
|
Sepi-23 |
0.39/0.49† |
n.d./n.d.‡ |
||||
|
Scap-24* |
0.41 |
n.d. |
0.41 |
n.d. |
||
|
CNS-35 |
0.36/0.85† |
n.d./n.d.‡ |
||||
|
Sepi-37 |
0.22/0.5† |
n.d./n.d.‡ |
||||
|
Scap-38* |
0.26 |
n.d. |
0.4 |
n.d. |
||
|
Eco-42* |
0.71/2.69† |
n.d./n.d.‡ |
||||
|
Pmir-62 |
0.28/0.68† |
n.d./w.b.‡ |
||||
|
Eco-64 |
|
|
0.11/0.54† |
n.d./n.d.‡ |
|
|
*Deceased.
#Subject 3, a separate bacteraemic episode.
†Two independent EDTA plasma collections within −30h<0<30h period relative to positive BC.
‡Two independent bacterial PCRs with five set of primers for each EDTA plasma collection.
Sepi - S. epidermidis; Scap - S. capitis; CNS - coagulase negative staphylococcus; Efu - E; faecium; Kpn - K. pneumoniae; Entc - Enterococcus spp; Psa - P. aeruginosa; Spn - S. pneumoniae; MRSA - methicillin resistant staphylococcus; MSSA - methicillin susceptible staphylococcus; Eco - E. coli; Pmir - P. mirabilis