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. 2022 Jul 7;323(3):F243–F254. doi: 10.1152/ajprenal.00065.2022

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Copyright © 2022 The Authors

Licensed under Creative Commons Attribution CC-BY 4.0. Published by the American Physiological Society.

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Figure 4. — Expression of CD80 and CD206 in F4/80^high and CD11b^high kidney macrophages after heat stress (HS). Using flow cytometry, we analyzed kidney macrophages by focusing on CD80 and CD206 expression in F4/80^high or CD11b^high macrophages. On day 1, the population of CD80-positive F4/80^high macrophages (A) and CD11b^high macrophages (B) was larger in the HS group than in the sham group. In the HS group, the population of CD206-positive F4/80^high macrophages (C) and CD11b^high macrophages (D) on day 7 was higher than that on day 0. On day 1, the ratio of CD80-positive to CD206-positive macrophages (the M1-to-M2 ratio) in the F4/80^high (E) and CD11b^high (F) macrophages were both significantly higher in the HS group than in the sham group, whereas in the HS group, they were significantly lower on day 7 than on day 1. Data represent means ± SE from 8 to 10 mice in each group. *P < 0.05; **P < 0.01.