Figure 1.

Chloroquine (CQ) exacerbates and 3-methyladenine (3-MA) has no effect on acute kidney injury development following high-dose cisplatin (CIS) treatment. The development of acute kidney injury in the presence of CQ or 3-MA autophagy inhibition was assessed 72 h after 20 mg/kg CIS or vehicle (VEH) treatment via blood urea nitrogen (BUN; A and D), urinary neutrophil gelatinase-associated lipocalin (NGAL; B and E), and kidney injury molecule-1 (Kim-1) mRNA expression in the kidney cortex normalized to β₂-microglobulin (C and F). Statistical differences were determined by one-way ANOVA followed by a Tukey’s post test. *Statistically different than VEH-treated mice. #Statistically different than CIS-treated mice. ^Statistically different than CQ- or 3-MA-treated mice. Data expressed as means ± SE; n = 2–10.