Figure 2.

CD45⁺ gating strategy. Representative images of flow cytometry gating performed on all samples are shown. Whole kidneys were homogenized before sample staining. One million events were recorded on a BD LSRFortessa. Gating was designed to eliminate dead cells and doublets before the CD45⁺ population was assessed. CD45⁺ gates were drawn based on unstained samples fun in each experiment.