Figure 5.

Chloroquine (CQ) and 3-methyladenine (3-MA) prevent repeated low-dose cisplatin (CIS)-induced kidney injury. The development of CIS-induced kidney injury in the presence of CQ or 3-MA autophagy inhibition was assessed after three or four weekly 9 mg/kg CIS or vehicle (VEH) treatments. Kidney injury was evaluated via blood urea nitrogen (BUN; A and D), urinary neutrophil gelatinase-associated lipocalin (NGAL; B and E), and kidney injury molecule-1 (Kim-1) mRNA expression in the kidney cortex normalized to β₂-microglobulin (C and F). Statistical differences were determined by one-way ANOVA followed by a Tukey’s post test. *Statistically different than VEH-treated mice. #Statistically different than CIS-treated mice. ^Statistically different than CQ- or 3-MA-treated mice. Data are expressed as means ± SE; n = 5–10.