Figure 6.

Chloroquine (CQ) and 3-methyladenine (3-MA) reduce signs of kidney inflammation following repeated low-dose cisplatin (CIS) treatment. CD45⁺ total immune cell infiltration in the kidney was measured via flow cytometry of whole kidney homogenates from mice treated with three or four weekly doses of 9 mg/kg CIS and either CQ (A) or 3-MA (B). Data are presented as percentages of positively labeled cells from the total number of single cells counted for each sample. mRNA expression of monocyte chemoattractant protein-1 (Mcp-1; C and F), tumor necrosis factor-α (Tnfα; D and G), and chemokine (C-X-C motif) ligand 1 (Cxcl1; E and H) were measured from renal cortex tissue. Expression was normalized to β₂-microglobulin. Statistical differences were determined by one-way ANOVA followed by a Tukey’s post test. *Statistically different than vehicle (VEH)-treated mice. #Statistically different than CIS-treated mice. ^Statistically different than CQ- or 3-MA-treated mice. Data are expressed as means ± SE; n = 5–10.