Figure 7.

Chloroquine (CQ) and 3-methyladenine (3-MA) decrease cisplatin (CIS)-induced kidney fibrosis. The development of cisplatin-induced kidney fibrosis in the presence of CQ or 3-MA autophagy inhibition was assessed after three or four weekly 9 mg/kg CIS or vehicle (VEH) treatments. Sirius red/fast green (A–D), Masson’s trichrome (E–H), and α-smooth muscle actin (α-SMA; I and J) immunohistochemistry staining of paraffin-embedded kidney sections were used to assess collagen deposition and myofibroblast accumulation. Quantification (C, D, G, and H) was calculated based on the optical density (OD) of positive staining as assessed with ImageJ. n = 5–10.