Figure 9.

Chloroquine (CQ) and 3-methyladenine (3-MA) alter expression of autophagy markers in repeated low-dose cisplatin (CIS) treatment. Western blot analysis was performed to assess relative protein levels of the indicated proteins in the renal cortex of mice treated with three or four weekly doses of 9 mg/kg CIS or vehicle (VEH) and either CQ (A) or 3-MA (D). Protein expression was assessed on the same gel run as shown in Fig. 8; therefore, the loading control images are the same. Densitometry was performed, and relative protein expression was graphed (B, C, and E–G). Each sample’s value is expressed relative to its control gene and normalized to the first VEH sample on the gel. n = 2–4. LC3, microtubule-associated protein light chain 3; p62, sequestosome 1/p62.