Figure 7.

Bladder PDGFRA⁺ fibroblasts express the universal fibroblasts genes Pi16 and Col15a1. A–D: fluorescent in situ hybridization with immunodetection was used to assess coexpression of Col15a1 and Pi16 in PDGFRA⁺ fibroblasts of the indicated regions of the bladder wall [suburothelial (SU), outer lamina propria (OLP), intermuscular (IM), and serosal (Se)]. Cell nuclei that were PDGFRA⁺;Col15a1⁺ are indicated by red arrowheads; cell nuclei that were PDGFRA⁺;Col15a1⁺;Pi16⁺ are indicated by white arrowheads. Cell nuclei that were PDGFRA^–;Col15a1⁺, likely smooth muscle cells, are indicated by orange arrowheads. D: a control incubation was performed in the absence of anti-PDGFRA antibody and using the three-plex negative control probe against Bacillus subtilis dapB. Otherwise, the sample was treated identically to experimental samples. Endolysosomes (EL) in the umbrella cell layer of the urothelium exhibited autofluorescence. All images are confocal micrographs and representative of images taken from two mice.