Figure 10.

Expression of CD34, PI16, and TNC in bladder fibroblasts. A: coexpression of PDGFRA, CD34, and TNC in the suburothelial region of the mouse bladder. The dashed boxed regions in the top images are magnified in the bottom images. Examples of cells (with visible nuclei) that coexpressed CD34 and PDGFRA are indicated by yellow arrows. B: expression of PDGFRA, CD34, and PI16 in the mouse bladder wall. PI16 was expressed by fibroblasts in the lamina propria, a fraction of fibroblasts in the innermost portions of the intermuscular region (i.e., the region closest to the urothelium), and in serosal fibroblasts. In the top images, nuclei of PDGFRA⁺; PI16⁺ serosal cells are indicated by cyan arrows and nuclei of PI16⁺; PDGFRA^– mesothelial cells are marked with magenta arrows. In the bottom images, nuclei of cells coexpressing PDGFRA, CD34, and PI16 are indicated by white arrows and nuclei of PDGFRA⁺, CD34⁺, PI16^– cells are marked with yellow arrows. All images are confocal micrographs and representative of images taken from three separate experiments each with two mice.