Table 1.
Antibodies used for flow cytometry
| Antigen | Fluorochrome | Concentration | Vendor | Catalog No. |
|---|---|---|---|---|
| CD8 | BB700 | 1:100 | BD | 566409 |
| CD3 | APC/Cy7 | 1:20 | BD | 557596 |
| CD4 | AF700 | 1:200 | BD | 557956 |
| CD64 | A647 | 1:20 | BD | 558539 |
| MHCII | PE/Cy7 | 1:800 | BioLegend/Thermo Fisher | 107630 |
| SigF | PE/CF594 | 1:20 | BD | 562757 |
| Ly6G | BV711 | 1:50 | BD | 563979 |
| CD45 | BV570 | 1:100 | BioLegend/Thermo Fisher | 103136 |
| Fixable viability dye | eFluor 506 | 1:1000 | eBioscience | 65086614 |
| Ly6C | BV421 | 1:200 | BioLegend/Thermo Fisher | 128032 |
| CD11b | BUV737 | 1:400 | BD | 612800 |
| CD19 | BUV563 | 1:50 | BD | 749028 |
| CD11c | BUV395 | 1:20 | BD | 564080 |
Note that the specificity of antibodies was verified based on information from datasheets provided by vendors.