. 2022 Jul 12;323(3):G205–G218. doi: 10.1152/ajpgi.00337.2021

Table 2.

Primer sequences used for RT-qPCR

Target Gene	Forward Sequence (5′–3′)	Reverse Sequence (5′–3′)
Tnf-α	`CCACCACGCTCTTCTGTCTA`	`AGGGTCTGGGCCATAGAACT`
Il1β	`GAAATGCCACCTTTTGACAGTG`	`TGGATGCTCTCATCAGGACAG`
Il2	`AGCAGCTGTTGATGGACCTA`	`CGCAGAGGTCCAAGTTCAT`
Il6	`GTTCTCTGGGAAATCGTGGA`	`TTCTGCAAGTGCATCATCGT`
Il10	`TGAATTCCCTGGGTGAGAAG`	`TGGCCTTGTAGACACCTTGG`
Il12p40	`GGAAGCACGGCAGCAGAATAA`	`CTTGAGGGAGAAGTAGGAATG`
Il15	`CATCCATCTCGTGCTACTTGTGTT`	`CATCTATCCAGTTGGCCTCTGT`
Il17	`AAGGCAGCAGCGATCATCC`	`GGAACGGTTGAGGTAGTCTGAG`
Il18	`GCCTCAAACCTTCCAAATCA`	`TGGATCCATTTCCTCAAAGG`
Nos2	`ACATCGACCCGTCCACAGTAT`	`CAGAGGGGTAGGCTTGTCTC`
Ifn-γ	`TGCTGATGGGAGGAGATGTCT`	`TTTCTTTCAGGGACAGCCTGTT`
Ccl2	`AGGTCCCTGTCATGCTTCTG`	`TCTGGACCCATTCCTTCTTG`
Ccl3	`TGAAACCAGCAGCCTTTGCTC`	`AGGCATTCAGTTCCAGGTCAGTG`
Ccl4	`AAACCTAACCCCGAGCAACA`	`CCATTGGTGCTGAGAACCCT`
Ccl5	`ACACCACTCCCTGCTGCTTT`	`GACTGCAAGATTGGAGCACTTG`
Ccl7	`AAGATCCCCAAGAGGAATCTCAAG`	`CAGACTTCCATGCCCTTCTTTG`
Cxcl1	`GACCATGGCTGGGATTCACC`	`CCAAGGGAGCTTCAGGGTCA`
Ccr2	`ACAGCTCAGGATTAACAGGGACTTG`	`ACCACTTGCATGCACACATGAC`
Ccr5	`AGGCCATGCAGGCAACAG`	`TCTCTCCAACAAAGGCATAGATGA`
Cxcr3	`CAGCCTGAACTTTGACAGAACCT`	`GCAGCCCCAGCAAGAAGA`
Gapdh	`AACTTTGGCATTGTGGAAGG`	`ACACATTGGGGGTAGGAACA`