Figure 1.

Effects of short-term exposure to a high-fat diet (HFD) on body weight and glucose tolerance. Male CD1 mice were fed chow or HFD (45% kcal from fat) for 2 wk. A: body weight change in chow (n = 8) and HFD (n = 10) mice. No significant differences were observed. B: oral glucose tolerance test (OGTT) in mice fed chow (n = 8) or HFD (n = 10). After an overnight fast, glucose levels (mg/dL) were measured in a fasting state at 5, 15, 30, 60, and 120 min after administration of 2 mg/kg body wt glucose solution (0.4 mg/mL) by oral gavage. Blood glucose relative to baseline was significantly higher in HFD compared with chow at 15 min (P = 0.0019, t = 3.766, df = 16), 30 min (P < 0.0001, t = 4.712, df = 16), and 60 min (P < 0.0001, t = 4.942, df = 16). ***P < 0.001, **P < 0.01 HFD vs. chow in two-way ANOVA followed by Bonferroni correction for multiple comparisons. C: baseline corrected area under the curve (AUC) of blood glucose levels calculated by subtracting baseline glucose (t = 0) from all timepoints for each mouse individually, followed by AUC calculation. AUC of blood glucose in HFD was significantly different from chow (P = 0.0014, U = 6). **P < 0.01 in Mann–Whitney U test. Data are mean ± SE, n = 8–10/group. Groups are representative of all chow and HFD groups used in this study.